![]() The goal of this collection is to give an overview of the new methodologies that can be used to perform human brain study from the macro to the micro-scale. Indeed, alteration of antigens, due to fixation and/or long storage, could prevent good immunostaining recognition. Such specimens present specific challenges that need to be solved in comparison to animal models: massive dimension of the specimen (up to several cm3), geometry, variability of post-mortem fixation conditions and storage, presence of blood inside the vessels, autofluorescence signals coming from lipofuscin-type pigments, and labeling. ![]() However, the analysis of the human brain is still in its infancy. These techniques already allowed us to map cell distribution or to reconstruct neuronal circuits in whole mouse brains. The combination of tissue preparation techniques, advanced optical microscopy, and big data analysis is revolutionizing the way of studying the brain anatomy.
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